Jackson二抗|315-036-046*
通過胃蛋白酶消化全IgG抗體產(chǎn)生F（ab'）2片段抗體，以除去大部分Fc區(qū)，同時留下一些鉸鏈區(qū)。 F（ab'）2片段具有通過二硫鍵連接在一起的兩個抗原結(jié)合Fab部分，因此它們是二價的。 平均分子量約為110kDa。 它們用于特定應用，例如避免二抗與活體細胞結(jié)合Fc受體或蛋白A或蛋白G。
基于免疫電泳和/或ELISA，抗體與小鼠IgG重鏈的Fc部分反應但不與小鼠免疫球蛋白的Fab部分反應。 未檢測到針對小鼠IgM或非免疫球蛋白血清蛋白的抗體。 已通過ELISA和/或固相吸附測試抗體以確保與人血清蛋白的小交叉反應，但其可與來自其他物種的免疫球蛋白交叉反應。
Jackson二抗|315-036-046*
Physical State: Freeze-dried solid
Storage:
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Suggested Working Concentration or Dilution Range:
1:500 - 1:5,000 for immunohisto/cytochemistry
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.